A QUANTITATIVE METHOD FOR MORPHOMETRIC ANALYSIS IN NEURONAL CELL-CULTURE - UNBIASED ESTIMATION OF NEURON AREA AND NUMBER OF BRANCH-POINTS

被引：23

作者：

VENTIMIGLIA, R ^{[1
]}

JONES, BE ^{[1
]}

MOLLER, A ^{[1
]}

机构：

[1] NEUROSEARCH AS,DK-2600 GLOSTRUP,DENMARK

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1995年 / 57卷 / 01期

关键词：

MORPHOMETRY; STEREOLOGY; CELL CULTURE; IN VITRO;

D O I：

10.1016/0165-0270(94)00126-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The morphology and fine structure of neurons in vivo as well as in vitro are influenced by a variety of cell-adhesion and extracellular matrix molecules and soluble growth factors. To examine the effects of such molecules, we have developed a new method for the quantitation of several parameters associated with the morphology of neurons in culture. Whereas methods which have been traditionally used to perform quantitative morphometric analysis of neurons in vitro are often time-consuming and subjective, the methods we describe provide a rapid, efficient, and unbiased approach to morphometric analysis of cultured neurons.

引用

页码：63 / 66

页数：4

共 14 条

[1]

Bottenstein J, 1979, Methods Enzymol, V58, P94

[2] OPTIMIZING SAMPLING EFFICIENCY OF STEREOLOGICAL STUDIES IN BIOLOGY - OR DO MORE LESS WELL [J].