INACTIVATION OF THROMBOXANE SYNTHASE WITH HYDROPEROXY-FATTY ACIDS

被引：2

作者：

HALL, ER

TOWNSEND, G

FRASIERSCOTT, KF

机构：

[1] UNIV HOUSTON CLEAR LAKE, SCH NAT & APPL SCI, DIV BIOL, BOX 306, HOUSTON, TX 77058 USA

[2] UNIV TEXAS, SCH MED, DEPT INTERNAL MED, HOUSTON, TX 77025 USA

[3] UNIV TEXAS, SCH MED, DEPT PHARMACOL, HOUSTON, TX 77025 USA

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1991年 / 42卷 / 01期

关键词：

D O I：

10.1016/0952-3278(91)90061-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine lung thromboxane synthase was immobilized on phenyl-Sepharose beads by absorption. The immobilized enzyme was catalytically active and synthesized both TXA2 and HHT. The structure-activity relationship of several hydroperoxy fatty acids and their ability to inactivate thromboxane synthase was investigated. Millimolar quantities of hydrogen peroxide and tert-butylperoxide were required to inactivate the enzyme; whereas micromolar quantities of C18 and C20 hydroperoxy fatty acids inactivated the enzyme. Pretreatment of the enzyme with long chain hydroperoxy-fatty acids resulted in a decreased synthesis fo both TXB2 and HHT.

引用

页码：19 / 22

页数：4

共 12 条

[1] IMMOBILIZATION OF CATALYTICALLY ACTIVE THROMBOXANE SYNTHASE [J].