ULTRASTRUCTURAL AND IMMUNOCYTOCHEMICAL STUDY OF P(1) PROTAMINE LOCALIZATION IN HUMAN TESTIS

被引：21

作者：

LESCOAT, D

BLANCHARD, Y

LAVAULT, MT

QUERNEE, D

LELANNOU, D

机构：

[1] Laboratoire D'histologie Et D'embryologie, Rennes

来源：

ANDROLOGIA | 1993年 / 25卷 / 02期

关键词：

SPERMIOGENESIS; P(1) PROTAMINE; MONOCLONAL ANTIBODY; TRANSMISSION ELECTRON MICROSCOPY; HUMAN;

D O I：

10.1111/j.1439-0272.1993.tb02689.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Two monoclonal antibodies (MAbs) directed against human protamine P1 were realized. Anti-P1 specificity was assessed by western-blot and confirmed by ELISA. Monoclonal antibody 97-3 was selected. Protamine P1 was specifically demonstrated in human testis by immunoelectron microscopy, using 97-3 MAb and an indirect post-embedding immunogold technique. Our results clearly demonstrated the precise time of appearance of P1 protamine in the nuclei of human spermatids. P1 first appeared in the nucleus of step 5 spermatids and its concentration was increased in steps 6-8 spermatids, cytoplasm was not labelled.

引用

页码：93 / 99

页数：7

共 28 条

[1] A MODEL FOR THE STRUCTURE OF CHROMATIN IN MAMMALIAN SPERM [J].