RABBIT FKBP59-HEAT SHOCK PROTEIN-BINDING IMMUNOPHILLIN (HBI) IS A CALMODULIN BINDING-PROTEIN

被引:78
作者
MASSOL, N [1 ]
LEBEAU, MC [1 ]
RENOIR, JM [1 ]
FABER, LE [1 ]
BAULIEU, EE [1 ]
机构
[1] MED COLL OHIO,DEPT OBSTET & GYNECOL & PHYSIOL,TOLEDO,OH 43699
关键词
D O I
10.1016/0006-291X(92)90448-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
FKBP59-HBI, a heat shock protein hsp90-binding immunophilin that was originally detected in heterooligomer forms of steroid receptors, is retained on Calmodulin (CAM)-Sepharose 4B in the presence of 2 mM Ca2+ and is eluted by EGTA, demonstrating a specific p59-CAM interaction. The p59 amino acid sequence reveals the presence of two putative CAM binding sites in α helix regions of the protein, as well as PEST sequences which are generally present in CAM-binding proteins. In vitro proteolysis by calpain II (a Ca2+-activated neutral protease), another feature of CAM-binding proteins, generates shorter peptides revealed by the mAb EC1, but not by the pAb 173 which recognizes the C-terminal of the protein. The potential function of CAM binding by the hsp90-binding immunophilin is discussed. © 1992.
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页码:1330 / 1335
页数:6
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