DETERMINATION OF BACTERIAL MURAMIC ACID BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY WITH NEGATIVE-ION DETECTION

被引：27

作者：

ELMROTH, I

FOX, A

LARSSON, L

机构：

[1] UNIV S CAROLINA,SCH MED,DEPT MICROBIOL & IMMUNOL,COLUMBIA,SC 29208

[2] UNIV LUND,DEPT MED MICROBIOL,S-22362 LUND,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 628卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)80335-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gas chromatographic-mass spectrometric method for the determination of trace amounts of muramic acid was developed. Muramic acid, a compound unique to bacterial peptidoglycan, not present elsewhere in nature, was measured as its trifluoroacetylated methyl glycoside, and in that form could be determined at low picogram levels (injected amount) when using negative-ion chemical ionization. The method included methanolysis, extraction, evaporation and acetylation. The presence of excess amounts of yeast in the samples did not interfere with the analysis. The method is rapid and simple and is useful for the determination of small amounts of peptidoglycan in complex environments.

引用

页码：93 / 102

页数：10

共 22 条

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