INACTIVATION OF POLYMERASE INHIBITORS FOR MYCOBACTERIUM-TUBERCULOSIS DNA AMPLIFICATION IN SPUTUM BY USING CAPTURE RESIN

被引：57

作者：

AMICOSANTE, M

RICHELDI, L

TRENTI, G

PAONE, G

CAMPA, M

BISETTI, A

SALTINI, C

机构：

[1] UNIV MODENA,DIPARTIMENTO SCI MED ONCOL & RADIOL,I-41100 MODENA,ITALY

[2] UNIV ROMA LA SAPIENZA,OSPED FORLANINI,DIPARTIMENTO SCI CARDIORESP,ROME,ITALY

[3] UNIV PISA,DIPARTIMENTO BIOMED,PISA,ITALY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 03期

关键词：

D O I：

10.1128/JCM.33.3.629-630.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).

引用

页码：629 / 630

页数：2

共 10 条

[1] LARGE-SCALE USE OF POLYMERASE CHAIN-REACTION FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN A ROUTINE MYCOBACTERIOLOGY LABORATORY
CLARRIDGE, JE
SHAWAR, RM
SHINNICK, TM
PLIKAYTIS, BB
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (08) : 2049 - 2056
[2] POLYMERASE CHAIN-REACTION AMPLIFICATION OF A REPETITIVE DNA-SEQUENCE SPECIFIC FOR MYCOBACTERIUM-TUBERCULOSIS
EISENACH, KD
CAVE, MD
BATES, JH
CRAWFORD, JT
[J]. JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (05) : 977 - 981
[3] RAPID DETECTION OF TUBERCULOUS AND NONTUBERCULOUS MYCOBACTERIA BY POLYMERASE CHAIN-REACTION AMPLIFICATION OF A 162 BP DNA FRAGMENT FROM ANTIGEN-85
FAUVILLEDUFAUX, M
VANFLETEREN, B
DEWIT, L
VINCKE, JP
VANVOOREN, JP
YATES, MD
SERRUYS, E
CONTENT, J
[J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1992, 11 (09) : 797 - 803
[4] DETECTION AND IDENTIFICATION OF MYCOBACTERIA BY AMPLIFICATION OF MYCOBACTERIAL DNA
HANCE, AJ
GRANDCHAMP, B
LEVYFREBAULT, V
LECOSSIER, D
RAUZIER, J
BOCART, D
GICQUEL, B
[J]. MOLECULAR MICROBIOLOGY, 1989, 3 (07) : 843 - 849
[5] RAPID DISCRIMINATION MYCOBACTERIUM-TUBERCULOSIS STRAINS BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS
LINTON, CJ
JALAL, H
LEEMING, JP
MILLAR, MR
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (09) : 2169 - 2174
[6] MANTERO G, 1991, CLIN CHEM, V37, P422
[7] DIRECT-DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN SPUTUM BY POLYMERASE CHAIN-REACTION AND DNA HYBRIDIZATION
NOLTE, FS
METCHOCK, B
MCGOWAN, JE
EDWARDS, A
OKWUMABUA, O
THURMOND, C
MITCHELL, PS
PLIKAYTIS, B
SHINNICK, T
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (07) : 1777 - 1782
[8] DETECTION AND IDENTIFICATION OF MYCOBACTERIA BY AMPLIFICATION OF A SEGMENT OF THE GENE CODING FOR THE 32-KILODALTON PROTEIN
SOINI, H
SKURNIK, M
LIIPPO, K
TALA, E
VILJANEN, MK
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) : 2025 - 2028
[9] CHARACTERIZATION OF A MYCOBACTERIUM-TUBERCULOSIS INSERTION-SEQUENCE, IS6110, AND ITS APPLICATION IN DIAGNOSIS
THIERRY, D
BRISSONNOEL, A
VINCENTLEVYFREBAULT, V
NGUYEN, S
GUESDON, JL
GICQUEL, B
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (12) : 2668 - 2673
[10] COMPARISON OF POLYMERASE CHAIN-REACTION AMPLIFICATION OF 2 MYCOBACTERIAL DNA-SEQUENCES, IS6110 AND THE 65KDA ANTIGEN GENE, IN THE DIAGNOSIS OF TUBERCULOSIS
WALKER, DA
TAYLOR, IK
MITCHELL, DM
SHAW, RJ
[J]. THORAX, 1992, 47 (09) : 690 - 694

← 1 →