COMBINED MICRODIALYSIS AND FOS IMMUNOHISTOCHEMISTRY FOR THE ESTIMATION OF DOPAMINE NEUROTRANSMISSION IN THE RAT CAUDATE-PUTAMEN

被引：23

作者：

MORELLI, M ^{[1
]}

CARBONI, E ^{[1
]}

COZZOLINO, A ^{[1
]}

TANDA, GL ^{[1
]}

PINNA, A ^{[1
]}

DICHIARA, G ^{[1
]}

机构：

[1] UNIV CAGLIARI, DEPT TOXICOL, VIALE A DIAZ 182, I-09126 CAGLIARI, ITALY

来源：

JOURNAL OF NEUROCHEMISTRY | 1992年 / 59卷 / 03期

关键词：

C-FOS; DOPAMINE; EARLY GENE; FOS; GBR-12909; MICRODIALYSIS; NEUROTRANSMISSION; D(1)-RECEPTOR;

D O I：

10.1111/j.1471-4159.1992.tb08359.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular dopamine (DA) concentrations estimated by transcerebral dialysis and D1-dependent c-fos expression, as demonstrated by Fos immunohistochemistry, were studied after blockade of DA reuptake by GBR-12909. Rats implanted with dialysis probes in the dorsal caudate-putamen did not show any Fos-positive neuronal labeling in the implanted area or in the rest of the caudate-putamen. Administration of GBR-12909 dose-dependently increased DA output in dialysates and resulted in the appearance in the caudate-putamen of Fos-positive neurons whose density was related to the dose of GBR- 12909 and to the increase in extracellular concentrations of DA. The D1 antagonist SCH-23390 blocked GBR-12909-induced activation of Fos while potentiating the stimulation of DA output. The results show that following blockade of DA reuptake by GBR-12909, the induction of Fos is related to stimulation of D1 receptors by extracellular DA. Combination of brain dialysis with Fos immunohistochemistry might provide a method for estimating the functional significance of extracellular DA as measured by brain microdialysis.

引用

页码：1158 / 1160

页数：3

共 8 条

[1] INVIVO BRAIN DIALYSIS OF NEUROTRANSMITTERS [J].