LC-MS AND MS-MS IN THE ANALYSIS OF RECOMBINANT PROTEINS

被引：7

作者：

COULOT, M ^{[1
]}

DOMON, B ^{[1
]}

GROSSENBACHER, H ^{[1
]}

GUENAT, C ^{[1
]}

MAERKI, W ^{[1
]}

MULLER, DR ^{[1
]}

RICHTER, WJ ^{[1
]}

机构：

[1] CIBA GEIGY AG,DEPT BIOTECHNOL,CH-4002 BASEL,SWITZERLAND

来源：

JOURNAL OF MOLECULAR STRUCTURE | 1993年 / 292卷

关键词：

D O I：

10.1016/0022-2860(93)80092-A

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Applicability and performance of electrospray ionization mass spectrometry (ESIMS) is demonstrated for protein analysis. ESIMS is applied in conjunction with on-line HPLC (LC-ESIMS) and direct tandem mass spectrometry (positive and negative ion mode ESIMS/MS) to the structural characterization of a recombinant protein (r-hirudin variant 1) and a congener phosphorylated at threonine45 (RP-1).

引用

页码：89 / 103

页数：15

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