CONVERSION OF SHORT-CHAIN CERAMIDES TO SHORT-CHAIN CERAMIDE GM3 IN B16 MELANOMA-CELLS

被引:13
作者
KOMORI, H [1 ]
ITO, M [1 ]
机构
[1] KYUSHU UNIV,FAC AGR,MARINE BIOCHEM LAB,HIGASHI KU,FUKUOKA 81281,JAPAN
关键词
SHORT-CHAIN CERAMIDE; C-2-CERAMIDE; C-6-CERAMIDE; ENDOGLYCOCERAMIDASE; GLYCOSPHINGOLIPID; GANGLIOSIDE;
D O I
10.1016/0014-5793(95)01137-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report that short-chain ceramide (Cer), C-2- and C-6-Cer, were immediately glycosylated and finally converted to short-chain Cer GM3 in B16 melanoma cells, By addition of either C-2- or C-6-Cer to a cell culture of B16 melanoma in the presence of [C-14]Gal, the radiolabeled precursor, was incorporated into each of two novel glycosphingolipids (GSLs) within 30 min along with synthesis of normal GSLs, These novel GSLs were identified as C-2-, C-6-Cer cerebrosides and C-2-, C-6-Cer GM3, respectively, In comparison with C-2-Cer, C-6-Cer was found to be much more efficiently converted to the GSLs, whereas no glycosylated sphingosine was detectable when it was added in place of short-chain Cer.
引用
收藏
页码:299 / 302
页数:4
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