POLYCOS VECTORS - A SYSTEM FOR PACKAGING FILAMENTOUS PHAGE AND PHAGEMID VECTORS USING LAMBDA-PHAGE PACKAGING EXTRACTS

被引：5

作者：

ALTINGMEES, MA ^{[1
]}

SHORT, JM ^{[1
]}

机构：

[1] STRATAGENE,LA JOLLA,CA 92037

来源：

GENE | 1993年 / 137卷 / 01期

关键词：

EXCISION; POLYMER; CONCATEMER; CLONING; PHAGE DISPLAY; EPITOPE; COS; ZAP;

D O I：

10.1016/0378-1119(93)90256-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A new class of hybrid vectors,'polycos' vectors, incorporate a phage lambda cos site and filamentous phage origin to allow high-efficiency cloning via in vitro lambda packaging extracts. The head-filling mechanism of cos site recognition by lambda. packaging proteins permits concatemers of several of these small cos-containing vectors to be packaged per phage particle. Excision of vector monomers after infection is accomplished via a lambda ZAP-like M13 excision process. This system has the advantage of adapting high-efficiency lambda packaging extracts to M13 and phagemid cloning vectors.

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页码：93 / 100

页数：8

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