ISOLATION OF THERMOSTABLE D-HYDANTOINASE-PRODUCING THERMOPHILIC BACILLUS SP SD-1

被引：21

作者：

LEE, SG

LEE, DC

SUNG, MH

KIM, HS

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT BIOTECHNOL,TAEJON 305701,SOUTH KOREA

[2] KIST,GERI,APPL MICROBIOL RES GRP,TAEJON 305600,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 05期

关键词：

D O I：

10.1007/BF01023326

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A thermophilic bacterium which showed highest thermostability and activity of the hydantoinase was isolated from 1,000 thermophiles and identified to be Bacillus sp. SD-1 according to morphological and physiological characteristics. The optimal growth temperature of the bacterium was about 60 degrees C. The hydantoinase of Bacillus sp. SD-1 was strictly D-specific, and optimal pH and temperature were determined to be about 8.0 and 70 degrees C, respectively. The D-hydantoinase was stable upto 70 degrees C, and half-life of the enzyme was about 20 min at 80 degrees C.

引用

页码：461 / 466

页数：6

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