THE IN-VITRO PRODUCTION OF AN ANTHOCYANIN FROM CALLUS-CULTURES OF OXALIS-LINEARIS

Callus growth and the production of anthocyanins were sustained on the salts and vitamins of Murashige and Skoog. Callus growth was stimulated at a concentration of 8-32 mu M alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Benzyladenine (BA) and zeatin at 8 mu M inhibited callus growth whereas isopentenyladenine (iP) stimulated callus growth. NAA repressed anthocyanin production with an increase in NAA from 8-32 mu M. Anthocyanin synthesis was promoted by an increase in 2,4-D from 0.5 to 2 mu M and decreased thereafter up to a concentration 32 mu M 2,4-D. A concentration of 8 mu M BA, thidiazuron and zeatin, respectively stimulated pigment production. Sucrose stimulated callus growth at 60 mM and pigment production at 120-360 mM.