KINETICS OF BILIRUBIN OXIDASE AND MODELING OF AN IMMOBILIZED BILIRUBIN OXIDASE REACTOR FOR BILIRUBIN DETOXIFICATION

被引:9
作者
MULLON, CJP
KLIBANOV, AM
LANGER, R
机构
[1] WHITAKER COLL HLTH SCI,HARVARD MIT DIV HLTH SCI & TECHNOL,BOSTON,MA 02139
[2] BOSTON CHILDRENS HOSP,DEPT SURG,BOSTON,MA
[3] MIT,DEPT APPL BIOL SCI,CAMBRIDGE,MA 02139
关键词
BIOTECHNOLOGY - CHROMATOGRAPHIC ANALYSIS - Column - FUNGI - Medical Applications - PROTEINS - Concentration;
D O I
10.1002/bit.260310605
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The unbound bilirubin concentration and the enzymatic rate of bilirubin degradation by bilirubin oxidase in bilirubin-serum albumin solutions have been investigated experimentally and theoretically. A stoichiometric bilirubin-serum albumin binding analysis shows that the unbound bilirubin concentration depends only on the molar ratio of the total bilirubin concentration to the total serum albumin concentration. From the theoretical analysis and the measured unbound bilirubin concentrations serum albumin may be modelled as a molecule having two binding sites, primary and secondary, with stoichiometric equilibrium constants of K//1 equals 6 multiplied by 10**7M** minus **1 and K//2 equals 4. 5 multiplied by 10**6 M** minus **1, respectively. The rate of total bilirubin degradation in bilirubin-serum albumin mixtures is zero order. An immobilized bilirubin oxidase reactor model, which shows good agreement with experimental bilirubin conversions, is presented.
引用
收藏
页码:536 / 546
页数:11
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