PROTEIN-KINASE-C IS INVOLVED IN THE EARLY SIGNALS OF INTERFERON-ALPHA BUT NOT OF INTERFERON-GAMMA IN U937 CELLS

被引：18

作者：

CSERMELY, P

BALINT, E

GRIMLEY, PM

ASZALOS, A

机构：

[1] UNIFORMED SERV UNIV HLTH SCI,F EDWARD HERBERT SCH MED,DEPT PATHOL,BETHESDA,MD 20814

[2] US FDA,DIV RES & TESTING,WASHINGTON,DC 20204

来源：

JOURNAL OF INTERFERON RESEARCH | 1990年 / 10卷 / 06期

关键词：

D O I：

10.1089/jir.1990.10.605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increasing interest is being focused on the role of protein kinase C (PKC) in the mode of action of interferons (IFNs). Here we report that IFN-alpha-induced a transient translocation of PKC from the cytosol to the particulate fraction of U937 cells. In contrast, after IFN-gamma-treatment, no significant change in PKC activity could be observed. IFN-induced changes in membrane potential were also examined by means of a potential sensitive oxonol dye and flow cytometry. Hyperpolarization was induced by both IFN-alpha and IFN-gamma. The protein kinase inhibitor H-7 blocked the hyperpolarization induced by IFN-alpha but not by IFN-gamma. These concordant results suggest that PKC is involved in the early signals of IFN-alpha but not of IFN-gamma in U937 cells.

引用

页码：605 / 611

页数：7

共 27 条

[1]

CLEMENS M.J., McNURLAN M.A., Regulation of cell proliferation and differentiation by interferon, Biochem. J, 226, pp. 345-360, (1985)

[2]

PESTKA S., LANGER J.A., ZOON K.C., SAMUEL C.E., Interferons and their actions, Annu. Rev. Biochem, 56, pp. 727-777, (1987)

[3]

BALKWILL F.R., Interferons, Lancet, i, pp. 1060-1063, (1989)

[4]

NISHIZUKA Y., Studies and perspectives of protein kinase C, Science, 233, pp. 305-312, (1986)

[5]

YAP W.H., TEO T.S., TAN Y.H., An early event in the interferon-induced transmembrane signaling process, Science, 234, pp. 355-358, (1986)

[6]

YAP W.H., TEO T.S., McCOY E., TAN Y.H., Rapid and transient rise in diacylglycerol concentration in Daudi cells exposed to interferon, Proc. Natl. Acad. Sci. USA, 83, pp. 7765-7769, (1986)

[7]

HAMILTON T.A., BECTON D.L., SOMERS S.D., GRAY P.W., ADAMS D.O., Interferon-γ modulates protein kinase C activity in murine peritoneal macrophages, J. Biol. Chem, 260, pp. 1378-1381, (1985)

[8]

ITO M., TAKAMI Y., TANABE F., SHIGETA S., TSUKUI K., KAWADE Y., Modulation of protein kinase C activity during inhibition of tumor cell growth by IFN-β and IFN-γ, Biochem. Biophys. Res. Commun, 150, pp. 126-132, (1988)

[9]

OSTROWSKI J., MEIER K.E., STANTON T.H., SMITH L.L., BOMSZTYK K., Interferon-γ and interleukin-1α induce transient translocation of protein kinase C activity to membranes in a B lymphoid cell line, J. Biol. Chem, 263, pp. 13786-13790, (1988)

[10]

PREMECZ G., MARKOVITS A., BAGI G., FARKAS T., FOLDES I., Phospholipase C and phospholipase A2 are involved in the antiviral activity of human interferon-α, FEBS Lett, 249, pp. 257-260, (1989)

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