INSULIN AND ISOPROTERENOL INDUCE PHOSPHORYLATION OF THE PARTICULATE CYCLIC GMP-INHIBITED, LOW KM CYCLIC-AMP PHOSPHODIESTERASE (CGI PDE) IN 3T3-L1 ADIPOCYTES

被引：6

作者：

VASTA, V

SMITH, CJ

CALVO, J

BELFRAGE, P

MANGANIELLO, VC

机构：

[1] NHLBI,CELLULAR METAB LAB,BLDG 10,ROOM SN-307,9000 ROCKVILLE PIKE,BETHESDA,MD 20892

[2] NICHHD,THEORET BIOL LAB,BETHESDA,MD 20892

[3] UNIV LUND,DEPT PHYSIOL CHEM,S-22101 LUND,SWEDEN

[4] NIH,BETHESDA,MD 20892

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80299-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cGI PDE in particulate fractions of differentiated adipocytes (but not control 3T3-L1 fibroblasts) was cross-reactive with a polyclonal antibody raised against the bovine adipose cGI PDE. The 3T3-L1 adipocyte cGI PDE is a 135 kDa protein which is phosphorylated in 32P-labeled cells in response to beta-agonist or insulin. These results indicate that the 3T3-L1 cGI PDE is similar in structure and hormonal regulation to the analogous enzyme in the rat adipocyte. © 1992 Academic Press, Inc.

引用

页码：1070 / 1075

页数：6

共 8 条

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SMITH, CJ ;

TORNQVIST, H ;

VASTA, V ;

BELFRAGE, P ;

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