BISPECIFIC IGA/IGM ANTIBODIES AND THEIR USE IN ENZYME-IMMUNOASSAY

被引：7

作者：

BEHRSING, O ^{[1
]}

KAISER, G ^{[1
]}

KARAWAJEW, L ^{[1
]}

MICHEEL, B ^{[1
]}

机构：

[1] MAX DELBRUCK CTR MOLEC MED, ROBERT ROSSLE STR 10, O-1115 BERLIN, GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 156卷 / 01期

关键词：

BISPECIFIC ANTIBODY; IGA/IGM HYBRID; ENZYME IMMUNOASSAY; HUMAN CHORIONIC GONADOTROPIN;

D O I：

10.1016/0022-1759(92)90012-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two hybrid hybridomas secreting polymeric bispecific antibodies to human chorionic gonadotropin and calf intestinal alkaline phosphatase were produced by fusion of IgA- and IgM-secreting mouse hybridomas. Both hybrid antibodies were purified from ascitic fluid by size exclusion chromatography. An IgM-like fraction was shown to exhibit bispecific activity. Bispecificity was completely lost following mild reduction and alkylation. Both bispecific antibodies were used to develop a sensitive enzyme immunoassay for hCG.

引用

页码：69 / 77

页数：9

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