MOLECULAR-CLONING AND EXPRESSION OF SUBUNIT-12 - A NON-MCP AND NON-ATPASE SUBUNIT OF THE 26-S-PROTEASE

被引：34

作者：

DUBIEL, W ^{[1
]}

FERRELL, K ^{[1
]}

DUMDEY, R ^{[1
]}

STANDERA, S ^{[1
]}

PREHN, S ^{[1
]}

RECHSTEINER, M ^{[1
]}

机构：

[1] UNIV UTAH,SCH MED,DEPT BIOCHEM,SALT LAKE CITY,UT 84132

来源：

FEBS LETTERS | 1995年 / 363卷 / 1-2期

关键词：

HUMAN 26 S PROTEASE; REGULATORY COMPLEX; SUBUNIT; 12; MOV-34;

D O I：

10.1016/0014-5793(95)00288-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding subunit 12 (S12) of human erythrocyte 26 S protease has been isolated, sequenced and expressed. The cDNA contains an open reading frame that encodes a 36.6 kDA protein 96% identical to mouse Mov-34 and 67% identical to its Drosophila melanogaster homolog. Based on the high degree of sequence identity between human S12, mouse and Drosophila Mov-34 proteins, we conclude that the Mov-34 gene product is a component of the 26 S protease. Antibodies produced against two S12 fragments, Met(1)-Tyr(95) (S12(f95)) and Met(1)-Leu(205) (S12(f205)), react with S12 transferred to nitrocellulose from SDS-PAGE, In contrast, after transfer from native gels, the epitope(s) recognized by anti-S12(f205) is exposed in the regulatory complex but appears to be masked when the regulatory complex associates with the multicatalytic protease.

引用

页码：97 / 100

页数：4

共 17 条

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