USING THE MICRONUCLEUS ASSAY TO DETECT GENOTOXIC EFFECTS OF METAL-IONS

被引：32

作者：

BERCES, J

OTOS, M

SZIRMAI, S

CRANEURUENA, C

KOTELES, GJ

机构：

[1] FREDERIC JOLIOT CURIE NATL RES INST RADIOBIOL & RADIOHYG,POB 101,H-1775 BUDAPEST,HUNGARY

[2] PAKS NUCL POWER PLANT,PAKS,HUNGARY

来源：

ENVIRONMENTAL HEALTH PERSPECTIVES | 1993年 / 101卷

关键词：

D O I：

10.2307/3431692

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The lymphocyte micronucleus assay was used to measure the average frequency of micronuclei in a population and thus assess genotoxic effects. Data from 174 persons glve an average value of 16.4 +/- 7.3, and a slight age-dependence was observed. To detect combined environmental mutagen injuries the micronucleus assay was used to study the effects of metal compounds. Cadmium ions increased the micronucleus frequency linearly after incubation with whole blood in vitro with 10(-6)-10(-3) M concentrations for 30 min. Similarly, a linear increase in micronucleus frequency was detected with 10(-3)-10(-1) M mercury ions. Concerning the biological effect of selenium, it was found that neither sodium selenite nor selenium dioxide induced increases at concentrations of 10(-7)-10(-6)M; 10(-5) M caused a slight increase; 10(-1) M, however destroyed the cells. These results suggest that the human lymphocyte micronucleus test can be used to assess genotoxic injuries due to environmental effects in human lymphocytes.

引用

页码：11 / 13

页数：3

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