EVALUATION OF A COMMERCIAL ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DIAGNOSIS OF PARATUBERCULOSIS IN DAIRY-CATTLE

被引：151

作者：

SWEENEY, RW

WHITLOCK, RH

BUCKLEY, CL

SPENCER, PA

机构：

[1] Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1995年 / 7卷 / 04期

关键词：

D O I：

10.1177/104063879500700411

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The performance of a commercially available ELISA for detection of antibodies to Mycobacterium paratuberculosis was evaluated using sera from 1,146 cows. Samples were from uninfected cattle, infected subclinical cattle shedding low numbers of organism in feces, subclinical heavy shedders, clinical cases, and randomly selected cattle in a slaughterhouse survey for paratuberculosis. The overall sensitivity of the test, using the manufacturer's recommended cutoff was 45% 1-4.8%, and the specificity was 99% +/- 0.9%. The ELISA result was significantly correlated with the number of colonies of M. paratuberculosis detected by fecal culturing. The sensitivity of the test was highest for clinical cases of paratuberculosis (87% +/- 8.4%), and lowest for subclinical, Light-shedding cattle (15% +/- 6.6%). Changing the cutoff point did not improve performance of the test. Evaluating ELISA results with a kinetics-based method reduced plate-to-plate variation in results but did not improve performance of the test based on receiver-operating characteristic curve analysis.

引用

页码：488 / 493

页数：6

共 15 条

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