THE APPLICATION OF RANDOM AMPLIFIED POLYMORPHIC DNA FOR SANDFLY SPECIES IDENTIFICATION

被引：35

作者：

ADAMSON, RE

WARD, RD

FELICIANGELI, MD

MAINGON, R

机构：

[1] UNIV LIVERPOOL,LIVERPOOL SCH TROP MED,WOLFSON UNIT MOLEC GENET,PEMBROKE PL,LIVERPOOL L3 5QA,ENGLAND

[2] UNIV CARABOBO,FAC CIENCIAS SALUD,BIOMED,MARACAY,VENEZUELA

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1993年 / 7卷 / 03期

关键词：

SANDFLY; DNA; RAPD PROFILE; PROBE; LEISHMANIASIS; PCR;

D O I：

10.1111/j.1365-2915.1993.tb00677.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

We have applied the recently developed Random Amplified Polymorphic DNA (RAPD) method to produce species-specific, DNA profiles for two sympatric. Venezuelan sandfly species, thought to be the vectors responsible for recent outbreaks of cutaneous and mucocutaneous leishmaniasis in the Andean State of Tachira. Moreover, within the profile, it was possible to identify a diagnostic DNA band for Lu.youngi of 0.32 kb. Results showed that the size of this diagnostic DNA band remained constant and did not vary with sex or geographical distribution.

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页码：203 / 207

页数：5

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