OMEGA-HYDROXYLATION OF DOCOSAHEXAENOIC ACID OR ARACHIDONIC-ACID IN HUMAN COLONIC WELL-DIFFERENTIATED ADENOCARCINOMA HOMOGENATE

被引：8

作者：

SHIMIZU, S

YAMANE, M

ABE, A

NAKAJIMA, M

SUGIURA, H

MIYAOKA, M

SAITOH, T

机构：

[1] TOKYO MED COLL, DEPT INTERNAL MED 4, SHINJUKU KU, TOKYO, JAPAN

[2] TOKYO MED COLL, DEPT BIOCHEM, SHINJUKU KU, TOKYO, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1256卷 / 03期

关键词：

OMEGA-HYDROXYDOCOSAHEXAENOIC ACID; OMEGA-HYDROXYEICOSATETRAENOIC ACID; OMEGA-OXIDATION; HUMAN COLONIC WELL DIFFERENTIATED ADENOCARCINOMA; LIQUID CHROMATOGRAPHY THERMOSPRAY MASS SPECTROMETRY;

D O I：

10.1016/0005-2760(95)00036-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human colonic well differentiated adenocarcinoma homogenate was incubated with NADPH and docosahexaenoic acid (22:6(n - 3)) or arachidonic acid (20:4(n - 6)) as a substrate. On a selected ion monitoring chromatogram obtained with reversed phase-high-performance liquid chromatography thermospray mass spectrometry, omega-hydroxydocosahexaenoic acid(omega-HDHE) or omega-hydroxyeicosatetraenoic acid (omega-HETE) from an incubation mixture of the homogenate was detected in significant amount, compared to that from a colonic region remote from the carcinoma. In contrast, epoxydocosapentaenoic acids and the dihydroxy derivatives from 22:6(n - 3) or epoxyeicosatrienoic acids and the dihydroxy derivatives from 20:4(n - 6) were detected in low amounts, compared to that from a colonic region remote from the carcinoma. The results suggest that highly active NADPH-dependent omega-oxidations of polyunsaturated fatty acids occur in colonic adenscarcinoma homogenate.

引用

页码：293 / 296

页数：4

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