CAMR, A NEGATIVE REGULATOR LOCUS OF THE CYTOCHROME-P-450CAM HYDROXYLASE OPERON

被引：32

作者：

KOGA, H ^{[1
]}

ARAMAKI, H ^{[1
]}

YAMAGUCHI, E ^{[1
]}

TAKEUCHI, K ^{[1
]}

HORIUCHI, T ^{[1
]}

GUNSALUS, IC ^{[1
]}

机构：

[1] UNIV ILLINOIS, DEPT BIOCHEM, URBANA, IL 61801 USA

来源：

JOURNAL OF BACTERIOLOGY | 1986年 / 166卷 / 03期

关键词：

D O I：

10.1128/jb.166.3.1089-1095.1986

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The camC gene specifies cytochrome P-450cam, and camD specifies the 5-exo-alcohol dehydrogenase. A 1.38-kilobase deletion from the insert results in the constitutive expression of genes camC and camD; transformation in trans restores the substrate control, indicating that camR is a negative regulator.

引用

页码：1089 / 1095

页数：7

共 43 条

[1] 14-ALPHA-DEMETHYLATION OF LANOSTEROL BY A RECONSTITUTED CYTOCHROME-P-450 SYSTEM FROM YEAST MICROSOMES [J].