EXPRESSION OF RICIN-B CHAIN IN SPODOPTERA-FRUGIPERDA

被引：22

作者：

FRANKEL, A ^{[1
]}

ROBERTS, H ^{[1
]}

AFRIN, L ^{[1
]}

VESELY, J ^{[1
]}

WILLINGHAM, M ^{[1
]}

机构：

[1] MED UNIV S CAROLINA,DEPT PATHOL,CHARLESTON,SC 29425

来源：

BIOCHEMICAL JOURNAL | 1994年 / 303卷

关键词：

D O I：

10.1042/bj3030787

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA encoding ricin B chain was derived from preproricin genomic DNA and ligated into the baculovirus transfer vector, pAcGP67A. Co-transfection of Spodoptera frugiperda Sf9 cells with BaculoGold DNA was followed by limiting dilution purification of recombinant baculovirus. Infection of SF9 cells at a multiplicity of infection of 1 in the presence of 25 mM lactose produced 3 mg/1 of soluble, glycosylated, immunoreactive with monoclonal and polyclonal antibodies to ricin B chain. The recombinant ricin B chain had similar lectin-binding activity to plant ricin B chain. The recombinant protein reassociated with ricin toxin. A chain similarly to ricin toxin B chain and the recombinant heterodimers had similar cell cytotoxicity to ricin.

引用

页码：787 / 794

页数：8

共 30 条

[1] CLONING AND EXPRESSION OF RECOMBINANT, FUNCTIONAL RICIN B-CHAIN
CHANG, MS
RUSSELL, DW
UHR, JW
VITETTA, ES
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) : 5640 - 5644
[2] DAVIS TR, 1993, IN VITRO CELL DEV-AN, V29A, P388
[3] ENDO Y, 1987, J BIOL CHEM, V262, P8128
[4] Frankel A E, 1993, Oncology (Williston Park), V7, P69
[5] FULTON RJ, 1986, J BIOL CHEM, V261, P5314
[6] GROSSBARD ML, 1992, CANCER RES, V52, P4200
[7] GRUENWALD S, 1993, BACULOVIRUS EXPRESSI
[8] GENOMIC CLONING AND CHARACTERIZATION OF A RICIN GENE FROM RICINUS-COMMUNIS
HALLING, KC
HALLING, AC
MURRAY, EE
LADIN, BF
HOUSTON, LL
WEAVER, RF
[J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (22) : 8019 - 8033
[9] HOUSTON LL, 1982, J BIOL CHEM, V257, P4147
[10] HSIEH P, 1984, J BIOL CHEM, V259, P2375

← 1 2 3 →