COMBINED LIQUID-LIQUID ELECTROEXTRACTION AND ISOTACHOPHORESIS AS A FAST ONLINE FOCUSING STEP IN CAPILLARY ELECTROPHORESIS

被引:50
作者
VANDERVLIS, E [1 ]
MAZEREEUW, M [1 ]
TJADEN, UR [1 ]
IRTH, H [1 ]
VANDERGREEF, J [1 ]
机构
[1] LEIDEN UNIV,LEIDEN AMSTERDAM CTR DRUG RES,CTR BIOPHARMACEUT SCI,DIV ANALYT CHEM,2300 RA LEIDEN,NETHERLANDS
关键词
D O I
10.1016/0021-9673(94)00776-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Combined liquid-liquid electroextraction and isotachophoresis as a fast on-line focusing step in capillary electrophoresis is described. Owing to the limited conductivity of the organic phase, high electric field strengths can be applied, resulting in high migration rates. Liquid-liquid electroextraction is used to focus the analyte ions from a large organic sample into a small volume in the terminating buffer zone, just above the liquid-liquid interface. Simultaneously, isotachophoresis is used to focus the analyte ions between the terminating buffer and the leading buffer. A steady state is reached within a few minutes, as isotachophoresis starts with a small sample volume. On-line capillary zone electrophoresis is used for the separation of the analytes. Propantheline, neostigmine, salbutamol and terbutaline were used as model compounds, and crystal violet was used to visualize the process for tuning the system. Concentration detection limits of pure solutions down to 10(-9)-10(-10) mol/l for the model compounds were obtained using simple UV absorbance detection.
引用
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页码:333 / 341
页数:9
相关论文
共 23 条
[1]   SOLVENT-EXTRACTION IN AN ELECTROSTATIC-FIELD [J].
BAILES, PJ .
INDUSTRIAL & ENGINEERING CHEMISTRY PROCESS DESIGN AND DEVELOPMENT, 1981, 20 (03) :564-570
[2]   ON-COLUMN SAMPLE CONCENTRATION USING FIELD AMPLIFICATION IN CZE [J].
CHIEN, RL ;
BURGI, DS .
ANALYTICAL CHEMISTRY, 1992, 64 (08) :A489-A496
[3]  
Deml M., 1988, ANAL ISOTACHOPHORESI
[4]   LARGE SAMPLE VOLUME PRESEPARATION FOR TRACE ANALYSIS IN ISOTACHOPHORESIS [J].
DOLNIK, V ;
DEML, M ;
BOCEK, P .
JOURNAL OF CHROMATOGRAPHY, 1985, 320 (01) :89-97
[5]  
DOLNIK V, 1990, J MICROCOLUMN SEP, V2, P127
[6]  
EVERAERTS FM, 1976, ISOTACHOPHORESIS
[7]   ON-COLUMN TRANSIENT AND COUPLED COLUMN ISOTACHOPHORETIC PRECONCENTRATION OF PROTEIN SAMPLES IN CAPILLARY ZONE ELECTROPHORESIS [J].
FORET, F ;
SZOKO, E ;
KARGER, BL .
JOURNAL OF CHROMATOGRAPHY, 1992, 608 (1-2) :3-12
[8]  
Foret F, 1990, J MICROCOLUMN SEP, V2, P229
[9]  
IYER PVR, 1974, P INT SOLV EXTR C, V2, P1029
[10]   ONLINE COUPLING OF CAPILLARY ISOTACHOPHORESIS WITH CAPILLARY ZONE ELECTROPHORESIS [J].
KANIANSKY, D ;
MARAK, J .
JOURNAL OF CHROMATOGRAPHY, 1990, 498 (01) :191-204