EFFICIENT HORMONE-INDUCIBLE PROTEIN FUNCTION IN XENOPUS-LAEVIS

被引：163

作者：

KOLM, PJ ^{[1
]}

SIVE, HL ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02142

来源：

DEVELOPMENTAL BIOLOGY | 1995年 / 171卷 / 01期

关键词：

D O I：

10.1006/dbio.1995.1279

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A major problem in analyzing gene function during Xenopus development has been the inability to induce gene expression in a temporally controlled manner. We have attempted to solve this problem with a system of hormone-activated protein function, using the myogenic gene MyoD as a paradigm. We show that microinjection of RNA for MyoD fused to the ligand-binding domain of either the estrogen or glucocorticoid receptor results in hormone-dependent activation of MyoD function, as assayed by ectopic induction of muscle-specific actin mRNA. Induction is tightly regulated in both isolated animal caps and intact embryos, with ectopic muscle-specific actin expression inducible after 2 hr of hormone treatment. Higher levels of MyoD-receptor fusion proteins that native MyoD protein are present in embryos, apparently a result of increased fusion protein stability. This is the first demonstration that hormone-inducible fusion proteins can work effectively in a complex embryo. (C) 1995 Academic Press, Inc.

引用

页码：267 / 272

页数：6

共 23 条

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