PURIFICATION AND CHARACTERIZATION OF EQUINE INFECTIOUS-ANEMIA VIRUS

被引：21

作者：

MATHEKA, HD ^{[1
]}

COGGINS, L ^{[1
]}

SHIVELY, JN ^{[1
]}

NORCROSS, NL ^{[1
]}

机构：

[1] CORNELL UNIV NEW YORK STATE COLL VET MED, DEPT PATHOL, RES LAB EQUINE INFECT DIS, ITHACA, NY USA

来源：

ARCHIVES OF VIROLOGY | 1976年 / 51卷 / 1-2期

关键词：

D O I：

10.1007/BF01317839

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

EIA [equine infectious anemia] virus was purified from equine fetal kidney cell cultures by PEG[polyethylene glycol]-precipitation, 2 sucrose-gradient sedimentations (5-30%) and (25-60%) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20.degree. C) of 1.162 and a sedimentation constant of S20W = 656. EM revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.

引用

页码：107 / 114

页数：8

共 16 条

[1]

Chervenka C H., 1969, A manual of methods for the analytical ultracentrifuge

[2]

COGGINS L, 1970, CORNELL VET, V60, P330

[3] DETECTION OF EQUINE INFECTIOUS ANEMIA VIRUS IN-VITRO BY IMMUNOFLUORESCENCE [J].