BEHAVIOR OF THE MAIZE TRANSPOSABLE ELEMENT AC IN ARABIDOPSIS-THALIANA

被引:102
作者
DEAN, C [1 ]
SJODIN, C [1 ]
PAGE, T [1 ]
JONES, J [1 ]
LISTER, C [1 ]
机构
[1] JOHN INNES CTR,SAINSBURY LAB,NORWICH NR4 7UJ,ENGLAND
关键词
D O I
10.1111/j.1365-313X.1992.00069.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The somatic and germinal activity of the maize transposable element, Ac, has been analysed in progeny of 43 transformants of A. thaliana using a streptomycin resistance assay to monitor Ac excision. The ability to assay somatic activity enabled, for the first time, a detailed analysis of Ac activity in individual A. thaliana seedlings to be made. The effects of T-DNA copy number, generation, dosage at each locus, flanking sequences and orientation of the element were compared. The most striking observation was the variability in Ac activity in genotypically identical individuals and the poor penetrance of the variegated phenotype. In general, increasing Ac dosage increased both somatic and germinal excision frequencies. The majority of families from individuals selected as inheriting an excision event carried transposed Ac elements re-integrated in different positions in the genome.
引用
收藏
页码:69 / 81
页数:13
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