INHIBITION OF ACTIVATED PROTEIN C AND ITS COFACTOR PROTEIN S BY ANTIPHOSPHOLIPID ANTIBODIES

被引：212

作者：

MALIA, RG ^{[1
]}

KITCHEN, S ^{[1
]}

GREAVES, M ^{[1
]}

PRESTON, FE ^{[1
]}

机构：

[1] UNIV SHEFFIELD,ROYAL HALLAMSHIRE HOSP,DEPT HAEMATOL,SHEFFIELD S10 2JF,S YORKSHIRE,ENGLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1990年 / 76卷 / 01期

关键词：

D O I：

10.1111/j.1365-2141.1990.tb07843.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Summary. We have investigated the effects of purified IgG fractions from plasma containing the lupus anticoagulant (LAC) and/or IgG anticardiolipin antibody (ACA) on the degradation of factor Va by an activated protein C‐protein S complex. Plasma samples from 10 patients were studied. LAC was detected by a Russell's Viper venom technique. ACA was determined by ELISA. IgG fractions were obtained from each plasma sample by protein A‐Sepharose fractionation. This fraction was shown to exhibit ACAILAC activity. Using purified activated protein C (APC), protein S and phosphati‐dylserine/phosphatidylcholine, factor Va degradation was assessed in the presence and absence of IgG fractions from LAC/ACA containing plasmas. After 2 min incubation the mean factor Va degradation by APC and protein S in the presence of IgG LAC/ACA fractions was 14% compared with 52% with normal IgG. A similar effect was seen when phospholipid was substituted by washed freeze‐thawed platelets. Experiments employing varying concentrations of protein S and phospholipid revealed marked differences in respect of the inhibitory specificity of the different antiphospholipid antibodies. These results indicate that antiphospholipid antibodies have an inhibitory effect on the activated protein C/protein S complex and provide some explanation for a relationship between antiphospholipid antibodies and thrombosis. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：101 / 107

页数：7

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