ROLE OF MYELIN PO PROTEIN AS A HOMOPHILIC ADHESION MOLECULE

PERIPHERAL nervous system myelin is an extension of the Schwann cell's plasma membrane that tightly enwraps axons in many layers and permits nerve impulses to be rapidly conducted. It is not known how these multiple membrane layers are held together in this compact form. Here we present evidence supporting the hypothesis that the extracellular leaflets of myelin1-4 are held together by the most abundant protein of myelin of the peripheral nervous system, Po, by homophilic interaction of its extracellular domains. Transfected Chinese hamster ovary cells expressing Po protein adhere to each other in suspension, to form large aggre-gates, whereas cells that are identical but which do not express Po do not. We also show that this aggregation is mediated by homophilic binding between Po-expressing cells and that the apposing plasma membranes of these cells specifically form desmo-somes, whereas control transfected cells do not. As the only difference between the two cell populations is the expression of Po, this protein is apparently responsible for the changes in morphology and adhesion in the cells that express it. The idea that Po is a homophilic adhesion molecule is supported by its inclusion in the immunoglobulin supergene family5, all members of which are involved in recognition and/or adhesion6. © 1990 Nature Publishing Group.