PURIFICATION OF MAIZE POLLEN EXINES AND ANALYSIS OF ASSOCIATED PROTEINS

被引：34

作者：

CHAY, CH ^{[1
]}

BUEHLER, EG ^{[1
]}

THORN, JM ^{[1
]}

WHELAN, TM ^{[1
]}

BEDINGER, PA ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT BIOL,CHAPEL HILL,NC 27599

来源：

PLANT PHYSIOLOGY | 1992年 / 100卷 / 02期

关键词：

D O I：

10.1104/pp.100.2.756

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Zea mays (maize) pollen exines have been purified with the use of differential centrifugation and sucrose gradients, followed by mild detergent and high salt treatment. The final exine fraction is highly purified from other organelles and subcellular structures as assayed by transmission electron microscopy. Using mature maize pollen as the starting material, 0.2 to 0.3% of the total pollen protein remained associated with the exine fraction throughout the purification. Seven abundant sodium dodecyl sulfate-extractable proteins are detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the final fraction. Amino acid analysis reveals that one of the proteins contains a substantial amount of hydroxyproline, a characteristic of some primary cell wall proteins. The amino acid composition of the 25-kD protein strongly implies that it is an arabinogalactan protein. When exines are purified from earlier pollen developmental stages, a subset of the proteins found in the mature pollen exine is seen.

引用

页码：756 / 761

页数：6

共 30 条

[1] ARABINOGALACTAN PROTEINS FROM STIGMAS OF NICOTIANA-ALATA [J].