COMPARISON OF MATRIX SOLID-PHASE DISPERSION (MSPD) WITH A STANDARD SOLVENT-EXTRACTION METHOD FOR SULFAMETHAZINE IN PORK MUSCLE USING HIGH-PERFORMANCE LIQUID AND THIN-LAYER CHROMATOGRAPHY

被引:17
作者
SHEARAN, P
OKEEFFE, M
SMYTH, MR
机构
[1] The National Food Centre, Dublin 15, Teagasc, Dunsinea, Castleknock
[2] School of Chemical Sciences, Dublin City University, Glasnevin
来源
FOOD ADDITIVES AND CONTAMINANTS | 1994年 / 11卷 / 01期
关键词
SULFAMETHAZINE; MUSCLE; MSPD; BACK EXTRACTION; HPLC; TLC;
D O I
10.1080/02652039409374197
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A rapid and simple extraction/clean-up procedure (matrix solid phase dispersion, MSPD) for the determination of sulphamethazine (SMZ) in pork muscle tissue is compared with a solvent extraction method. Extracts of samples fortified with SMZ or of incurred samples were found to be free from interfering compounds when chromatographed using HPLC or TLC separation systems. Recovery of SMZ from fortified samples is greater than 80% and residue levels of incurred samples found using the MSPD procedure compare favourably with results obtained using the solvent extraction method. Use of aqueous back extraction of SMZ from dichloromethane is also reported as an alternative step to solvent evaporation for ease of use in both the laboratory and in industry (i.e. at slaughter plants).
引用
收藏
页码:7 / 15
页数:9
相关论文
共 17 条
[1]  
Barker S.A., Long A.R., Short C.R., Isolation of drug residues from tissues by solid phase dispersion, Journal of Chromatography, 475, pp. 353-361, (1989)
[2]  
Conway B., Determination of sulphadimidine in animal feeds by high performance liquid chromatography, Analyst, 113, pp. 1397-1400, (1988)
[3]  
Cox B.L., Krzeminski L.K., High pressure liquid chromatographic determination of sulphamethazine in pork tissues, Journal of the Association of Official Analytical Chemists, 65, pp. 1311-1315, (1982)
[4]  
Haagsma N., Van Der Water C., Rapid determination of five sulphonamides in swine tissue by high-performance liquid chromatography, Journal of Chromatography, 333, pp. 256-261, (1985)
[5]  
Haagsma N., Nooteboom R.J., Gortemaker B.G.M., Maas M.J., A rapid sample preparation method for the determination of sulphamethazine in swine tissues by high performance liquid chromatography, Zeitschrift Fur Lebensmittel-Untersuchung Und -Forschung, 181, pp. 194-197, (1985)
[6]  
Haagsma N., Pluijmaker H.G.J.H., Aerts M.M.L., Beek W.J.M., Rapid sample preparation methods for analysis of residues of sulphamethazine and its β-acetyl and desamino metabolites in swine tissue by HPLC, Biomedical Chromatography, 2, pp. 41-45, (1987)
[7]  
Horie M., Saito K., Hoshino Y., Nose N., Hamada N., Nakazawa H., Identification and determination of sulphamethazine and A β-acetylsulphamethazine in meat by high performance liquid chromatography with photodiode-array detection, Journal of Chromatography, 502, pp. 371-378, (1990)
[8]  
Houglum J.E., Larson R.D., Neal R.M., Liquid chromatographic determination of sulphamethazine in feeds, Journal of the Association of Official Analytical Chemists, 71, pp. 1054-1056, (1988)
[9]  
Long A.R., Hsieh L.C., Malbrough M.S., Short C.R., Barker S.A., Method for the isolation and liquid chromatographic determination of furazolidone in milk, Journal of Agricultural and Food Chemistry, 38, pp. 430-432, (1990)
[10]  
Long A.R., Hsieh L.C., Malbrough M.S., Short C.R., Barker S.A., Method for the isolation and liquid chromatographic determination of chloramphenicol in milk, Journal of Agricultural and Food Chemistry, 38, pp. 427-429, (1990)