DRUG-INDUCED TOLERANCE TO ALLOGRAFTS IN MICE .9. ESTABLISHMENT OF COMPLETE CHIMERISM BY ALLOGENEIC SPLEEN-CELL TRANSPLANTATION FROM DONORS MADE TOLERANT TO H-2-IDENTICAL RECIPIENTS

Graft-versus-host reaction (GVH) after allogeneic spleen cell transplantation was completely suppressed in an H-2-matched murine combination (AKR/J Sea [H-2k] .fwdarw. lethally irradiated C3H/He Slc [H-2k]) by pretreatment of the donors with recipient spleen cell antigen plus cyclophosphamide (CP). Irradiated recipients receiving cells became chimeric. In contrast to the H-2 matched combination, lethal GVH reaction could not be prevented in an H-2-mismatched fully allogeneic combination (C57BL/6 Cr Slc [H-2b] .fwdarw. lethally irradiated C3H/He Slc [H-2k]) by pretreatment of the donors. The results suggest that the effectors responsible for the donors with allogeneic recipient spleen cells plus CP in the H-2-matched combination, but donor pretreatment failed to abrogate GVH reaction in the H-2-mismatched combination.