DETERMINATION OF CISPLATIN IN HUMAN PLASMA BY HPLC WITH A GLASSY CARBON-BASED WALL-JET AMPEROMETRIC DETECTOR

Previous voltametric experiments have shown the electrooxidation of cisplatin (cis-diamminedichloroplatinum II, CDDP) and its hydrolysis products at the glassy carbon electrode. Taking into account the advantages of the oxidative detection mode(rather than reduction) and by using an easy to handle wall-jet configuration, the sensitive HPLC determination of cis-platin has been successfully performed in biological samples. Several counter-ions generally employed in ion-pair chromatography have been investigated in order to optimize the analysis of CDDP in terms of separation efficacy, retention time and compatibility with the electrochemical detection mode.The cationic detergent cetyltrimethylammonium chloride(CTACl) has been found to be the most appropriate ion-pairing reagent for the determination of intact CDDP in spiked plasma.The limit of detection was 30 ng/ml and 150 ng/ml in aqueous and plasma samples, respectively. The recovery of cis-DDP in plasma was 86 +/- 5 % . At a concentration of 10 mug/ml, the coefficient of variation was 6 % (within-day) .