COMPARISON OF POLYMERASE CHAIN-REACTION WITH VIRUS ISOLATION AND HEMAGGLUTINATION ASSAYS FOR THE DETECTION OF CANINE PARVOVIRUSES IN FECAL SPECIMENS

被引：79

作者：

MOCHIZUKI, M ^{[1
]}

SANGABRIEL, MC ^{[1
]}

NAKATANI, H ^{[1
]}

YOSHIDA, M ^{[1
]}

机构：

[1] UNIV TOKYO,FAC MED,ANIM CTR BIOMED RES,BUNKYO KU,TOKYO 113,JAPAN

来源：

RESEARCH IN VETERINARY SCIENCE | 1993年 / 55卷 / 01期

关键词：

D O I：

10.1016/0034-5288(93)90035-E

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A polymerase chain reaction (PCR) assay, which specifically amplifies the capsid gene of canine parvovirus (CPV), was compared as a diagnostic method for detecting CPV in faeces, with virus isolation (VI) on Crandell feline kidney (CRFK) or Madin-Darby canine kidney (MDCK) cells, and a faecal haemagglutination (HA) assay confirmed by inhibition with a CPV-specific antiserum. Although a false-negative result was obtained in one of 59 faecal samples (1.7 per cent) tested by the PCR assay, it was as sensitive as the VI assay using MDCK cells, and more sensitive than the VI assay using CRFK cells or the HA assay. These results indicate that the PCR assay may be useful as a routine diagnostic method for detecting CPV in faecal specimens.

引用

页码：60 / 63

页数：4

共 10 条

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