SEQUENTIAL INVOLVEMENT OF NK CELLS AND CD8(+) T-CELLS IN GRANULOMA-FORMATION OF RHODOCOCCUS AURANTIACUS-INFECTED MICE

被引:12
作者
ASANO, M [1 ]
NAKANE, A [1 ]
KOHANAWA, M [1 ]
MINAGAWA, T [1 ]
机构
[1] HIROSAKI UNIV,SCH MED,DEPT BACTERIOL,HIROSAKI,AOMORI 036,JAPAN
关键词
IFN-GAMMA; GRANULOMA FORMATION; RHODOCOCCUS AURANTIACUS;
D O I
10.1111/j.1348-0421.1995.tb02234.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We investigated the effect of in vivo administration of antibodies against T-cell subsets and natural killer (NK) cells on endogenous gamma interferon (IFN-gamma) production and granuloma formation in Rhodococcus aurantiacus-infected mice. High titers of endogenous IFN-gamma were detected in the extracts of the livers and spleens during 24 hr of the infection, reaching the peak at 8 hr, and the IFN-gamma, production was reduced by in vivo administration of anti-NK 1.1 monoclonal antibody (MAb) or antibody against asialo GM1(+) cells. Endogenous IFN-gamma declined until 2 days of the infection, then reappeared from 1 week and peaked at 3 weeks. Endogenous IFN-gamma at 1 and 3 weeks was reduced by in vivo administration of anti-CDS MAb, but not by anti-CD4 MAb or anti-NK 1.1 MAb. Granulomatous lesions in the livers and spleens began to appear from 1 week of the infection and developed in 3 weeks. In vivo administration of rat anti-IFN-gamma MAb reduced the development of granulomas. Tn addition, granuloma formation was reduced by depletion of NK cells prior to the infection or depletion of CD8(+) T cells at 1 week of the infection. Based on these findings, it is presumed that the biphasic production of IFN-gamma is attributable to NK cells in the early phase of the infection and CD8(+) T cells in the phase of granuloma formation, and that granuloma formation is regulated by NK cells and CD8(+) T cells through the secretion of endogenous IFN-gamma.
引用
收藏
页码:499 / 507
页数:9
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