CRYSTALLIZATION AND PRELIMINARY-X-RAY INVESTIGATION OF FACTOR-D OF HUMAN-COMPLEMENT

被引：6

作者：

NARAYANA, SVL

KILPATRICK, JM

ELKABBANI, O

BABU, YS

BUGG, CE

VOLANAKIS, JE

DELUCAS, LJ

机构：

[1] UNIV ALABAMA,SCH OPTOMETRY,BIRMINGHAM,AL 35294

[2] UNIV ALABAMA,CTR MACROMOLEC CRYSTALLOG,BIRMINGHAM,AL 35294

[3] UNIV ALABAMA,DEPT MED,DIV CLIN IMMUNOL & RHEUMATOL,BIRMINGHAM,AL 35294

[4] BIOCRYST INC,BIRMINGHAM,AL 35205

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 01期

关键词：

CRYSTALLIZATION; FACTOR-D; COMPLEMENT; SERINE PROTEASE; DIFFRACTION;

D O I：

10.1016/0022-2836(91)90851-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human factor D, an essential enzyme of the alternative pathway of complement activation, has been crystallized. Crystals were grown by vapor diffusion using polyethylene glycol 6000 and NaCl as precipitants. The factor D crystals are triclinic and the space group is P1 with unit cell dimensions a = 40·8 A ̊, b = 64·7 A ̊, c = 40·3 A ̊, α = 101·0°, β = 109·7°, γ = 74·3°. The unit cell contains two molecules of factor D related by a non-crystallographic 2-fold axis. The crystals grow to dimensions of 0·8 mm × 0·5 mm × 0·2 mm within five days, are stable in the X-ray beam and diffract beyond 2·5 Å. © 1991.

引用

页码：1 / 3

页数：3

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