DETECTION AND EVALUATION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) IN RAT-TISSUE BY AN IMPROVED IMMUNOHISTOCHEMICAL PROCEDURE

被引：101

作者：

FOLEY, JF

DIETRICH, DR

SWENBERG, JA

MARONPOT, RR

机构：

[1] National Institute of Environmental Health Sciences, Research Triangle Park, NC, 27709

[2] University of North Carolina at Chapel Hill, Chapel Hill, NC

来源：

JOURNAL OF HISTOTECHNOLOGY | 1991年 / 14卷 / 04期

关键词：

FORMALIN FIXATION; PARAFFIN PROCESSING; PROLIFERATING CELL NUCLEAR ANTIGEN; SUPER SENSITIVE BIOTIN-STREPTAVIDIN;

D O I：

10.1179/his.1991.14.4.237

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Proliferating cell nuclear antigen (PCNA), an endogenous cell replication marker, was detected by an improved immunohistochemical procedure (super sensitive biotin-streptavidin), in formalin fixed, paraffin embedded tissue. Small intestine, liver, kidney, testis, and esophagus from rats were stained for the detection and evaluation of PCNA as a suitable marker for cell proliferation. Different patterns of staining that are believed to correlate with the individual phases of the cell cycle (G1, S, G2, M, G0) were recognized. PCNA offers an alternative to established cell labeling techniques for investigating cell proliferation.

引用

页码：237 / 241

页数：5

共 16 条

[1]

Wright N.A., Appleton D.R., The metaphase arrest technique: A Critical Review, Cell Tissue Kinet, 13, pp. 643-663, (1980)

[2]

Tannock I.F., A comparison of the relative efficiencies of various metaphase arrest agents, Exp Cell Res, 47, pp. 345-356, (1967)

[3]

Simpson-Herren L., Autoradiographic techniques for measurement ofthe labeling index, Techniques in Cell Cycle Analysis, pp. 1-29, (1987)

[4]

Brown J.M., Oliver R., A new method of estimating the cell cycle time in epithelial tissues of long generation time, Cell Tissue Kinet, 1, pp. 11-21, (1968)

[5]

Bravo R., Frank R., Blundell P.A., Macdonald-Bravo H., Cy-clin/PCNA is the auxiliary protein of DNA polymerase delta, Nature, 326, pp. 515-517, (1987)

[6]

Bauer G.A., Burgers P., The yeast analog of mammalian cy-clin/proliferating cell nuclear antigen interacts with mammalian DNA polymerase delta, Proc Natl Acad Sci USA, 85, pp. 7506-7510, (1988)

[7]

Celis J.E., Madsen P., Celis A., Et al., Cyclin (PCNA, auxiliary protein of DNA polymerase delta) is a central component of the pathway(s) leading to DNA replication and cell division, FEBS Lett, 220, pp. 1-7, (1987)

[8]

Takasaki Y., Deng J.S., Tan E.M., A nuclear antigen associated with cell proliferation and blast transformation. Its distribution in synchronized cells, J Exp Med, 154, pp. 1899-1909, (1981)

[9]

Celis J., Celis A., Cell cycle dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: Subdivision of S phase, Proc Natl Acad Sci USA, 82, pp. 3262-3266, (1985)

[10]

Kurki P., Vanderlaan M., Dolbeare et al: Expression of proliferating cell nuclear antigen (PCNA)/Cyclin during the cell cycle, Exp Cell Res, 166, pp. 209-219, (1986)

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