SALT-INDUCED REFOLDING OF MYOGLOBIN AT ACIDIC PH - MOLECULAR-PROPERTIES OF A PARTLY FOLDED INTERMEDIATE

被引：27

作者：

BISMUTO, E ^{[1
]}

SIRANGELO, I ^{[1
]}

IRACE, G ^{[1
]}

机构：

[1] NAPLES UNIV,DIPARTIMENTO BIOCHIM & BIOFIS,VIA COSTANTINOPOLI 16,I-80138 NAPLES,ITALY

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1992年 / 298卷 / 02期

关键词：

D O I：

10.1016/0003-9861(92)90458-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular properties of the salt-induced partly folded acidic state of apomyoglobin as well as myoglobin were investigated by fluorescence and circular dichroism of the extrinsic fluorophore 1,8-anilinonaphthalenesulfonate. The occurrence of a fluctuating tertiary structure ("molten globule") at acidic pH in the presence of salt was suggested by the disappearance of the dichroic activity of the fluorophore bound to the partly folded protein. Moreover, the structure of the intermediate is not influenced by the presence of heme, thus suggesting that heme is not crucial in the early stage of myoglobin folding. © 1992.

引用

页码：624 / 629

页数：6

共 36 条

[1] FLUORESCENCE STUDIES OF APLYSIA AND SPERM WHALE APOMYOGLOBINS [J].

ANDERSON, S ;

BRUNORI, M ;

WEBER, G .

BIOCHEMISTRY, 1970, 9 (24) :4723-+

[2] EQUILIBRIUM EVIDENCE OF NON-SINGLE STEP TRANSITION DURING GUANIDINE UNFOLDING OF APOMYOGLOBINS [J].

BALESTRIERI, C ;

COLONNA, G ;

GIOVANE, A ;

IRACE, G ;

SERVILLO, L .

FEBS LETTERS, 1976, 66 (01) :60-64

[3] UNFOLDING PATHWAY OF MYOGLOBIN - EVIDENCE FOR A MULTISTATE PROCESS [J].

BISMUTO, E ;

COLONNA, G ;

IRACE, G .

BIOCHEMISTRY, 1983, 22 (18) :4165-4170

[4]

Cantor CR, 1980, BIOPHYSICAL CHEM 2

[5]

CHRISTENSEN H, 1991, EUR BIOPHYS J, V19, P221, DOI 10.1007/BF00183530

[6] EFFECT OF EVOLUTION ON HOMOLOGOUS PROTEINS - COMPARISON BETWEEN CHROMOPHORE MICROENVIRONMENTS OF ITALIAN WATER BUFFALO (BOS-BUBALUS L) AND SPERM WHALE APOMYOGLOBIN [J].

COLONNA, G ;

IRACE, G ;

PARLATO, G ;

ALOJ, SM ;

BALESTRIERI, C .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 532 (02) :354-367

[7] HEME AND CYSTEINE MICRO-ENVIRONMENTS OF TUNA APOMYOGLOBIN - EVIDENCE OF 2 INDEPENDENT UNFOLDING REGIONS [J].

COLONNA, G ;

BALESTRIERI, C ;

BISMUTO, E ;

SERVILLO, L ;

IRACE, G .

BIOCHEMISTRY, 1982, 21 (02) :212-215

[8]

DAYHOFF MO, 1972, ATLAS PROTEIN SEQUEN, V5

[9]

DOLGIKH DA, 1985, EUR BIOPHYS J BIOPHY, V13, P109, DOI 10.1007/BF00256531

[10]

FINK AL, 1990, CURRENT RES PROTEIN

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