SOXRS GENE INCREASED THE LEVEL OF ORGANIC-SOLVENT TOLERANCE IN ESCHERICHIA-COLI

被引：42

作者：

NAKAJIMA, H

KOBAYASHI, M

NEGISHI, T

AONO, R

机构：

[1] Department of Bioengineering, Tokyo Institute of Technology, Yokohama 227

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 07期

关键词：

D O I：

10.1271/bbb.59.1323

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli strain JA300 grows in the presence of n-hexane, but not in the presence of cyclohexane. We isolated a 10.5-kb DNA fragment that provided cyclohexane tolerance on a multi-copy plasmid, from the chromosomal DNA of JA300. In this fragment, there were found C-terminal 10-amino acids truncated soxR (soxR') and soxS which control the superoxide response regulon genes. Characterization of subclones found that both the soxR' and overexpression of the soxS increased the levels of organic solvent tolerance in several E. coli strains.

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页码：1323 / 1325

页数：3

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