DEVELOPMENT OF THE H-POINT STANDARD ADDITIONS METHOD FOR THE USE OF SPECTROFLUOROMETRY AND SYNCHRONOUS SPECTROFLUOROMETRY

The basis of the H-point standard additions method (HPSAM) is developed for the use of fluorimetric and synchronous spectrofluorimetric measurements. The method permits the transformation of the indeterminate error resulting from the presence of an interference in the determination of an analyte into a systematic constant error that can be evaluated and permits the determination of the analyte free from bias error. Some examples are presented. Phenol fluorescence calibrations in water have a high interference from the Raman emission band of the solvent, The HPSAM permits the elimination of this interference, because the method can eliminate the blank bias error. Resolution of highly overlapped species, such as phenol-o-cresol mixtures, can be carried out with the method. In order to show this possibility, synchronous spectrofluorimetry was chosen to eliminate the blank signal.