SEPARATION OF PHOTOSYNTHETIC PIGMENTS AND THEIR PRECURSORS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A PHOTODIODE-ARRAY DETECTOR

被引：32

作者：

SCHOEFS, B

BERTRAND, M

LEMOINE, Y

机构：

[1] UNIV LILLE 1, PLANT CYTOPHYSIOL & PHYCOL LAB SN2, F-59655 VILLENEUVE DASCQ, FRANCE

[2] NATL INST TECH & SCI SEA, F-50110 TOURLAVILLE, FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 692卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01066-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase HPLC method is described for the separation of photosynthetic pigments, allowing the rapid and efficient separation of protochlorophyll(ide)s, chlorophyll(ide)s and cis- and trans-carotenoid isomers. The pigment contents of non-illuminated and illuminated bean leaves were determined and compared. Both sample types present the same carotenoid pattern. The main carotenoids are always trans-violaxanthin, trans-lutein and beta-carotene. The tetrapyrrole pigment contents are very different. Non-illuminated leaves mainly contain protochlorophyllide and protochlorophyll esters whereas illuminated leaves contain mainly chlorophylls.

引用

页码：239 / 245

页数：7

共 52 条

[1]

ADAMSON H, 1990, PROGR PHOTOSYNTHESIS, V3, P687

[2]

[Anonymous], 1984, OFFICIAL METHODS ANA

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