DIFFERENTIAL SUPPRESSION OF BACKGROUND MAMMALIAN LYSOSOMAL BETA-GALACTOSIDASE INCREASES THE DETECTION SENSITIVITY OF LACZ-MARKED LEUKEMIC-CELLS

被引：12

作者：

HENDRIKX, PJ

MARTENS, ACM

VISSER, JWM

HAGENBEEK, A

机构：

[1] ERASMUS UNIV ROTTERDAM,INST HEMATOL,ROTTERDAM,NETHERLANDS

[2] NEW YORK BLOOD CTR,NEW YORK,NY 10021

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 222卷 / 02期

关键词：

D O I：

10.1006/abio.1994.1516

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the detection of Escherichia coli beta-galactosidase-expressing leukemic cells in ex vivo bone marrow samples. 4-Methylumbelliferyl-beta-D-galactopyranoside is used as a substrate in a kinetic assay. D-Galactose is used to suppress endogenous lysosomal beta-galactosidase activity, yielding a sixfold increase in sensitivity. With this assay, the detection limit is one leukemic cell per 10(4) normal bone marrow cells. (C) 1994 Academic Press, Inc.

引用

页码：456 / 460

页数：5

共 11 条

[1]

CASADABAN MJ, 1983, METHOD ENZYMOL, V100, P293

[2]

EUSTICE DC, 1991, BIOTECHNIQUES, V11, P739

[3] IMPROVED FACS-GAL - FLOW CYTOMETRIC ANALYSIS AND SORTING OF VIABLE EUKARYOTIC CELLS EXPRESSING REPORTER GENE CONSTRUCTS [J].