Thioredoxin-linked peroxidase from human red blood cell: Evidence for the existence of thioredoxin and thioredoxin reductase in human red blood cell

被引：64

作者：

Cha, MK ^{[1
]}

Kim, IH ^{[1
]}

机构：

[1] PAI CHAI UNIV, DEPT BIOCHEM, TAEJON 302735, SOUTH KOREA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 217卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2856

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A thiol-dependent antioxidant protein (HRPRP) was previously reported as a predominant antioxidant protein in human red blood cell (RBC). The analysis of amino acid sequence of HRPRP with those of human PRP-like gene products indicates that HRPRP is identical to brain PRP (HPRP). This protein act as a peroxidase linked to thioredoxin (Trx)/thioredoxin reductase (TR). Until now, there was no evidence for Trx/TR system in RBC. The existence of the Trx/TR system in RBC was immunologically determined. A 58-kDa protein showing TR activity was partially purified from human RBC and characterized. Our results reveal that HRPRP act as a new type of peroxidase supported by Trx/TR system in human RBC. (C) 1995 Academic Press, Inc. Press. Inc.

引用

页码：900 / 907

页数：8

共 21 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

CHAE HZ, 1993, J BIOL CHEM, V268, P16815

[3]

CHAE HZ, 1994, J BIOL CHEM, V269, P27670

[4] OVOTHIOLS AS BIOLOGICAL ANTIOXIDANTS - THE THIOL-GROUPS OF OVOTHIOL AND GLUTATHIONE ARE CHEMICALLY DISTINCT [J].

HOLLER, TP ;

HOPKINS, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (14) :4837-4838

[5] THIOREDOXIN [J].

HOLMGREN, A .

ANNUAL REVIEW OF BIOCHEMISTRY, 1985, 54 :237-271

[6]

HOLMGREN A, 1977, J BIOL CHEM, V252, P4600

[7] INDUCTION OF AN ANTIOXIDANT PROTEIN OF SACCHAROMYCES-CEREVISIAE BY O-2,FE-3+, OR 2-MERCAPTOETHANOL [J].

KIM, IH ;

KIM, K ;

RHEE, SG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6018-6022

[8]

KIM KW, 1988, J BIOL CHEM, V263, P4704

[9] INHIBITION OF METAL-CATALYZED OXIDATION SYSTEMS BY A YEAST PROTECTOR PROTEIN IN THE PRESENCE OF THIOREDOXIN [J].

KWON, SJ ;

PARK, JW ;

CHOI, WK ;

KIM, IH ;

KIM, KW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 201 (01) :8-15

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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