ESCHERICHIA-COLI-CELLS RESISTANT TO THE DNA GYRASE INHIBITOR, CIPROFLOXACIN, OVERPRODUCE A 60-KD PROTEIN HOMOLOGOUS TO GROEL

Using a variety of mutagenic methods, we have generated a series of ciprofloxacin‐resistant mutants derived from Escherichia coli strains which overproduce the DNA gyrase A protein. Many of these mutants are found to overexpress a 60 kD protein which is shown to be highly homologous in terms of N‐terminal a mi no acid sequence to the E. coli heat‐shock protein, GroEL. Other evidence confirms that the 60 kD protein is unrelated to DNA gyrase and is similar, but not identical, to GroEL. Copyright © 1990, Wiley Blackwell. All rights reserved