MICROSCOPIC EVALUATION OF THE DANSYL CHLORIDE TEST

被引：12

作者：

PIERARD, GE ^{[1
]}

机构：

[1] STATE UNIV LIEGE,DEPT DERMATOPATHOL,B-4000 LIEGE,BELGIUM

来源：

DERMATOLOGY | 1992年 / 185卷 / 01期

关键词：

STRATUM-CORNEUM; SOAP; DANSYL CHLORIDE; SKIN SURFACE BIOPSY; MORPHOMETRY;

D O I：

10.1159/000247400

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The classical dansyl chloride test relies on visual assessment of the extinction of fluorescence in time. We introduce a microscopic and morphometric rating of this test. We show that soaps may remove the fluorescing dye from corneocytes. Therefore caution should be taken in interpreting fluorescence extinction only as an estimate of the stratum corneum renewal.

引用

页码：37 / 40

页数：4

共 16 条

[1]

DUGAIN F, 1974, REV ALUM, V3, P197

[2] A FLUORESCENCE PHOTOGRAPHIC PHOTOMETRIC TECHNIQUE TO ASSESS STRATUM-CORNEUM TURNOVER RATE AND BARRIER FUNCTION INVIVO [J].

FINLAY, AY ;

MARSHALL, RJ ;

MARKS, R .

BRITISH JOURNAL OF DERMATOLOGY, 1982, 107 (01) :35-41

[3] STRATUM-CORNEUM LIPID REMOVAL BY SURFACTANTS - RELATION TO INVIVO IRRITATION [J].

FROEBE, CL ;

SIMION, FA ;

RHEIN, LD ;

CAGAN, RH ;

KLIGMAN, A .

DERMATOLOGICA, 1990, 181 (04) :277-283

[4] DANSYL CHLORIDE .2. EFFECTS OF ANATOMICAL SITE AND PATCH TESTING TIME [J].

HARRISON, LB ;

HARRISON, D ;

KEELER, SJ .

JOURNAL OF TOXICOLOGY-CUTANEOUS AND OCULAR TOXICOLOGY, 1986, 5 (01) :55-62

[5]

JANSEN LH, 1974, BRIT J DERMATOL, V90, P9

[6]

JOHANNESSON A, 1978, ACTA DERM-VENEREOL, V58, P76

[7] THE RATE OF FORMATION OF CORNEOCYTE LAYERS AFTER STRIPPING THE NON-INVOLVED PSORIATIC SKIN AND ITS RELATION TO PARAKERATOSIS AND EPIDERMAL ENZYME LEVELS [J].

JOHANNESSON, A ;

HAMMER, H .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1980, 74 (04) :226-229

[8] MODIFIED TECHNIQUE (USING POLYESTER TAPE) OF SKIN SURFACE BIOPSY - ITS INTEREST FOR INVESTIGATION OF ATHLETES FOOT [J].

LACHAPELLE, JM ;

GOUVERNEUR, JC ;

BOULET, M ;

TENNSTEDT, D .

BRITISH JOURNAL OF DERMATOLOGY, 1977, 97 (01) :49-52

[9] A SIMPLIFIED METHOD FOR MEASUREMENT OF DESQUAMATION USING DANSYL CHLORIDE FLUORESCENCE [J].

MARKS, R ;

BLACK, D ;

HAMAMI, I ;

CAUNT, A ;

MARSHALL, RJ .

BRITISH JOURNAL OF DERMATOLOGY, 1984, 111 (03) :265-270

[10] SKIN SURFACE BIOPSY - IMPROVED TECHNIQUE FOR EXAMINATION OF HORNY LAYER [J].

MARKS, R ;

DAWBER, RPR .

BRITISH JOURNAL OF DERMATOLOGY, 1971, 84 (02) :117-&

← 1 2 →