INDUCTION OF MICRONUCLEI BY HTLV-I TAX - A CELLULAR-ASSAY FOR FUNCTION

被引：79

作者：

MAJONE, F ^{[1
]}

SEMMES, OJ ^{[1
]}

JEANG, KT ^{[1
]}

机构：

[1] NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892

来源：

VIROLOGY | 1993年 / 193卷 / 01期

关键词：

D O I：

10.1006/viro.1993.1145

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cellular chromosomal damage is ubiquitously seen in HTLV-I-transformed lymphocytes. It is also characteristic of cells that have been exposed to mutagens. A sensitive measurement for mutagen-induced DNA damage is the formation of micronuclei in treated cells. Because current evidence suggests that HTLV-I Tax is etiologically linked to transformation, we tested for its activity in inducing micronuclei. We show here that transfection into cells of a Tax-producing plasmid rapidly induced the formation of micronuclei. This effect cooperated with that of a mutagen (mitomycin C) and was correlated with the inherent trans-activation capacity of Tax. These findings suggest that a commonly used mutagen assay could be a quick biological test for putatively oncogenic proteins. © 1993 Academic Press, Inc.

引用

页码：456 / 459

页数：4

共 27 条

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