EXPLORING THE MYCOPLASMA-CAPRICOLUM GENOME - A MINIMAL CELL REVEALS ITS PHYSIOLOGY

被引：62

作者：

BORK, P

OUZOUNIS, C

CASARI, G

SCHNEIDER, R

SANDER, C

DOLAN, M

GILBERT, W

GILLEVET, PM

机构：

[1] MASSACHUSETTS GEN HOSP, BOSTON, MA 02114 USA

[2] HARVARD UNIV, BOSTON, MA 02138 USA

[3] GEORGE MASON UNIV, FAIRFAX, VA 22030 USA

来源：

MOLECULAR MICROBIOLOGY | 1995年 / 16卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1995.tb02321.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the analysis of 214 kb of the parasitic eubacterium Mycoplasma capricolum sequenced by genomic walking techniques. The 287 putative proteins detected to date represent about half of the estimated total number of 500 predicted for this organism. A large fraction of these (75%) can be assigned a likely function as a result of similarity searches. Several important features of the functional organization of this small genome are already apparent. Among these are (i) the expected relatively large number of enzymes involved in metabolic transport and activation, for efficient use of host cell nutrients; (ii) the presence of anabolic enzymes; (iii) the unexpected diversity of enzymes involved in DNA replication and repair; and (iv) a sizeable number of orthologues (82 so far) in Escherichia coli. This survey is beginning to provide a detailed view of how M. capricolum manages to maintain essential cellular processes with a genome much smaller than that of its bacterial relatives.

引用

页码：955 / 967

页数：13

共 56 条

[1]

Adams M.D., Kerlavage A.R., Fields C., Venter J.C., 3400 new expressed sequence tags identify diversity of transcripts in human brain, Nature Genetics, 4, pp. 256-267, (1993)

[2]

Alonso C., Shirahiga K., Oganawana N., Molecular cloning, genetic characterization, and DNA sequence analysis of the recM region of B. subtilis, Nucl Acids Res, 18, pp. 6771-6777, (1990)

[3]

Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J., Basic local alignment search tool, J Mol Biol, 215, pp. 403-410, (1990)

[4]

Bairoch A., Boeckmann B., The SWISS‐PROT protein sequence databank, Nucl Acids Res, 21, pp. 3093-3096, (1993)

[5]

Blattner F.R., Burland V., Plunkett G., Sofia H.J., Daniels D.L., Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.9 minutes, Nucl Acids Res, 21, pp. 5408-5417, (1993)

[6]

Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E., Comprehensive sequence analysis of the 182 ORFs of yeast chromosome III, Prot Sci, 1, pp. 1677-1690, (1992)

[7]

Bork P., Ouzounis C., Sander C., From genome sequences to protein function, Curr Opin Struct Biol, 4, pp. 393-403, (1994)

[8]

Claverie J.-M., States D., Information enhancement methods for large scale sequencing analysis, Comp Chem., 17, pp. 191-201, (1993)

[9]

Daniels D.L., Plunkett, Burland V., Blattner F., Analysis of the E. coli genome: DNA sequence of the region from 84.5 to 86.5 minutes, Science, 257, pp. 771-778, (1992)

[10]

Doolittle R.F., Bork P., Evolutionarily mobile protein modules, Sci Am, 269, 4, pp. 50-56, (1993)

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