DEVELOPMENTAL EXTINCTION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II GENE-EXPRESSION IN PLASMACYTES IS MEDIATED BY SILENCING OF THE TRANSACTIVATOR GENE CIITA

被引:138
作者
SILACCI, P [1 ]
MOTTET, A [1 ]
STEIMLE, V [1 ]
REITH, W [1 ]
MACH, B [1 ]
机构
[1] UNIV GENEVA,SCH MED,CMU,DEPT GENET & MICROBIOL,L JEANTET LAB MOLEC GENET,CH-1211 GENEVA 4,SWITZERLAND
关键词
D O I
10.1084/jem.180.4.1329
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Constitutive major histocompatibility complex (MHC) class II gene expression is tightly restricted to antigen presenting cells and is under developmental control. Cells of the B cell lineage acquire the capacity to express MHC class II genes early during ontogeny and lose this property during terminal differentiation into plasma cells. Cell fusion experiments have suggested that the extinction of MHC class II expression in plasma cells is due to a dominant repression, but the underlying mechanisms are not understood. CIITA was recently identified as an MHC class II transactivator that is essential for MHC class II expression in B lymphocytes. We show here that inactivation of MHC class II genes in plasmocytes is associated with silencing of the CIITA gene. Moreover, experimentally induced expression of CIITA in plasmocytes leads to reexpression of MHC class II molecules to the same level as that observed on B lymphocytes. We therefore conclude that the loss of MHC class II expression observed upon terminal differentiation of B lymphocytes into plasmocytes results from silencing of the transactivator gene CIITA.
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页码:1329 / 1336
页数:8
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