THE RIBOSOME AT IMPROVED RESOLUTION - NEW TECHNIQUES FOR MERGING AND ORIENTATION REFINEMENT IN 3D CRYOELECTRON MICROSCOPY OF BIOLOGICAL PARTICLES

被引：358

作者：

PENCZEK, PA ^{[1
]}

GRASSUCCI, RA ^{[1
]}

FRANK, J ^{[1
]}

机构：

[1] SUNY ALBANY,DEPT BIOMED SCI,ALBANY,NY 12222

来源：

ULTRAMICROSCOPY | 1994年 / 53卷 / 03期

关键词：

D O I：

10.1016/0304-3991(94)90038-8

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Cryo-electron microscopy of single biological particles opens up new possibilities for structure analysis: the particle can be reconstructed in its native shape and internal features are preserved. To take advantage of these possibilities we have developed new methods of data collection and image processing and we have applied them to the 70S Escherichia coli ribosome. A method of orientation search is proposed, which makes it possible to relate random-conical data sets to one another even if they are collected from low-tilt micrographs. A technique for 3D alignment of projections is described acid applied to the single-micrograph 3D reconstruction.

引用

页码：251 / 270

页数：20

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