QUANTITATIVE-ANALYSIS OF THE 2 MACROPHAGE-COLONY-STIMULATING FACTOR MESSENGER-RNA EXPRESSED IN A HUMAN STROMAL CELL-LINE BY REVERSE TRANSCRIPTION POLYMERASE CHAIN-REACTION (RT-PCR)

被引：13

作者：

OHTSUKI, T

IKEDA, M

HATAKE, K

TOMIZUKA, H

HOSHINO, Y

SUZU, S

HARIGAYA, K

MOTOYOSHI, K

MIURA, Y

机构：

[1] JICHI MED SCH,DEPT INTERNAL MED,DIV HEMATOL,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

[2] MORINAGA MILK IND CO LTD,BIOCHEM RES LAB,KANAGAWA,JAPAN

[3] CHIBA UNIV,SCH MED,DEPT PATHOL 1,CHIBA 280,JAPAN

[4] NATL DEF MED COLL,DEPT INTERNAL MED 3,SAITAMA,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1222卷 / 02期

关键词：

CSF; M-; MESSENGER-RNA; ALTERNATIVE SPLICING; PCR; RT-PCR; INTERFERON-GAMMA; (HUMAN);

D O I：

10.1016/0167-4889(94)90162-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We established a quantitative analysis system for 4.0 kb and 1.6 kb macrophage colony-stimulating factor (M-CSF) mRNA, using reverse transcription-polymerase chain reaction. Using this system, we performed quantitative analysis of the two mRNAs expressed in the human stromal cell line, KM102, in the resting condition and when stimulated by various concentrations of interferon-gamma (IFN-gamma). The expression of 1.6 kb M-CSF mRNA was more efficiently stimulated by IFN-gamma than that of 4.0 kb M-CSF mRNA. The alternative splicing of a single M-CSF gene has been shown to generate several M-CSF proteins with different localization; we believe that molecular analysis of the transcription products by this system is important to better understand the physiological significance of the different species of M-CSF derived from each mRNA.

引用

页码：141 / 146

页数：6

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